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Preparation and long-term cultivation of porcine tracheal and lung organ cultures by alternate exposure to gaseous and liquid medium phases. Characterisation of guinea pig precision-cut lung slices: comparison with human tissues. Natural innate cytokine response to immunomodulators and adjuvants in human precision-cut lung slices. interstitial lung diseases), our data might contribute to the development of further subacute or even CRD ex-vivo disease models and new therapeutic substances for these, particularly including slowly metabolized therapeutics. Corresponding to these earlier PCLS models of slowly progressive pulmonary diseases (e.g.
DÃ¶cker was teaching urban planning and reconstruction from 1947 to 1960 as professor at the (then) Technical University of Stuttgart. DÃ¶cker was in charge of construction management of the Weissenhof Estate in Stuttgart (a housing built for the Stuttgart Construction Expo in 1927) where architect Heinz Rasch also worked.
Subsequently, we verify the transferred address (by the customer login in the customer’s online banking). For this purpose, you have to redirect the customer to be verified from your website to our input form. Notification password: Automatically generated, is required for the hash value calculation for the notification Project password: Automatically generated, is required for the hash value calculation for the initial call and return Maximum number of failed verifications per user: Specifies how often a user can execute the verification per day.
According to the psychiatrist and art collector Ottomar Domnick, Benner was someone â€œâ€¦ who gave women eccentric hairstyles, dyed hair in bizarre colours, and generally lived a life that didnâ€™t fit in with the well-behaved city of Stuttgart at all.â€ (Domnick, 1989, S. In 1968 Herbert Bayer took over the design of the Stuttgart exhibition â€ž50 Jahre Bauhausâ€œ (50 years Bauhaus). The close connection with the Rasch family is reflected in Liloâ€™s family portrait from 1952, where Lilo depicts the shellac coated plaster sculpture â€œKÃ¤mmendeâ€ (Girl Combing Her Hair) (WV 51 A1) created by Braun in 1930, presumably recording the inaugural visit at the Baum residence.
in a manufacturing environment, so as to execute positioning, movement or co-ordinated operations by means of programme data in numerical form CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS SHAPING OR JOINING OF PLASTICS; SHAPING OF SUBSTANCES IN A PLASTIC STATE, IN GENERAL; AFTER- TREATMENT OF THE SHAPED PRODUCTS, e.g.
Towards a basic endoscopic assessment of swallowing in acute stroke – development and evaluation of a simple dysphagia score. Randomised comparison of percutaneous endoscopic gastrostomy and nasogastric tube feeding in patients with persisting neurological dysphagia.
Also of note, there was no overgrowth of fibroblasts observable at any time point. The number of pneumocytes, on the other hand, seemed to be reduced during long-term cultivation.
21-24 are arranged in data display lines 51-54, which are connected at one end to a machine control 81, a hot runner control 82, a hot runner control 83 and a temperature control unit control 84 and are connected at the other end to a main data line 55. 11, that is connected to a control device 31 via a data line 71.
The observations made in single cell culture systems that upon stimulation TNF-Î± secretion by macrophages decreases per se over time, with or without medium supplements, indicate that altered TNF-Î± secretion in long-term PCLS culture may be a problem of cultivation in general rather than specifically of PCLS. The presented study investigates on the one hand different vitality markers, completing the recently published work of Temann et al., and on the other hand functionality markers of long-term cultivated human PCLS, which to our knowledge have not been described before. Further, the here presented histology data shows no overgrowth of fibroblasts during the serum-free cultivation of human PCLS, which is in agreement with the work of Siminski et al.
The data protection declaration of the SchÃ¼chtermann-Klinik Bad Rothenfelde is based on the terms used by the European legislator for the adoption of the General Data Protection Regulation (GDPR). However, Internet-based data transmissions may in principle have security gaps, so absolute protection may not be guaranteed.
PCLS were washed thoroughly before cultivation in DMEM (2 slice per 500 Î¼l) under normal immersion culture conditions (37 Â°C, 5% CO 2 , and 100% air humidity) for up to 15Â days. 8 mm) were sliced in cold EBSS using a Krumdieck tissue slicer (Alabama Research and Development, Munford, AL, USA) into approx. Lung donors for this study were diverse regarding age, gender, medical history and cause of resection. In the context of CRD research models, PCLS have been used as a supportive tool after disease establishment in-vivo .
This enables us to study HRV induced immune responses in the human lung microenvironment. These data were confirmed by increased protein levels of pro-inflammatory and anti-viral cytokines induced by HRV, e.g. At 1d and 3d post infection (p.i.) tissue vitality, viral load and cytokine levels were measured and transcriptomic analyses upon RNA isolation from PCLS were performed. Human PCLS containing airways were inoculated with HRV1B, UV-inactivated HRV, medium or HRV in presence of Rupintrivir. Fraunhofer Institute for Toxicology and Experimental Medicine, Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease (BREATH) research network, Member of the Cluster of Excellence Regenerative Biology to Reconstructive Therapy (REBIRTH), Hannover, Germany